DNA conformational changes Observed via tethered-particle method

Dots: experimental data for the probabibility distribution function of bead excursion at three different tether lengths. Lines: our theoretical prediction, made with zero free fit parameters.

Nelson: NBIC and NSF DMR

Objective: develop new, noninvasive methods to visualize DNA conformational changes in real time, in physiological conditions.

Our results open the way to use this method to monitor gene regulatory processes, such as DNA looping, in real time.

References: PC Nelson, D. Brogioli, C. Zurla, D. D. Dunlap, and L. Finzi, submitted to JMB.

Dots: experimental data for the probabibility distribution function of bead excursion at three different tether lengths. Lines: our theoretical prediction, made with zero free fit parameters.