Development of Multi-Probe Mechanical and Structural Spectroscopy of Single-Molecules

H. Shuman, D. Discher, J. Schotland, Y.E. Goldman

Global objectives and specific goals of the project: Multi-parameter structural dynamics probed simultaneously by AFM or Optical Trap and TIRF Microscopy

Scientific strategy, approach and uniqueness: Make optimal use of complementary techniques and synergies

Conclusion and impact: Specialized TIRF microscopes objective have high infrared throughput, making them ideal for combined instrumentation. Optical trap manipulation of cytoskeletal filament, actin, into the evanescent wave of the TIRF microscope shows controllable approach to within 100 nm of the surface.

References: (too new)